Training Requirements for Personnel Working in Research Wet Labs

A safe laboratory is one in which the PI or Laboratory Supervisor ensures the following training for personnel:

Methods and observations that may be used to detect the presence or release of any hazardous materials in use (such as monitoring conducted by the employer, continuous monitoring devices, visual appearance or presence of odors when being released, etc.).
The physical and health hazards of any hazardous materials in use or procedures used in the work area.
The measures workers can take to protect themselves from these hazards, to include specific procedures the employer has implemented to protect workers from exposure to hazardous materials, such as appropriate work practices, emergency procedures, and personal protective equipment to be used.
The applicable details of the written laboratory-specific Chemical Hygiene Plan/IBC Registration/IACUC Protocol/Radiation Safety Protocol, etc.

The trainings listed below are required of all personnel working in wet labs conducting research at the University of Kentucky.

Other trainings may be added for or by the lab that are provided by internal sources (i.e., UK Biosafety Training, UK Radiation Safety Training, other focused modules online or in-person), (i.e., hands-on training of specific procedures in the lab by the PI or Lab Supervisor, UK EHS online modules or in-person session) or external (i.e. CITI) sources.

Documentation of this training shall be maintained in the laboratory's records and available upon request at time of inspection by internal university or external intenties.(e.g., Chemical Hygiene Plan)

Please be aware that students under the age of 18 who are not enrolled at the university are exempted from the requirement for completion of Fire Extinguisher training.

Job Activity	Safety Training Requirement	Training Method – Course Name	Training Frequency
Works in a laboratory	Fire Extinguisher Training Laboratory Specific Training	Fire Extinguisher Training https://ehs.uky.edu/classes/main.php?cClassID=13 Laboratory Specific Training Laboratory-Specific Training Form	Once Once, a training record for every individual in the lab must be documented and kept on site in the lab.
Works with chemicals in the laboratory	Chemical Hygiene Awareness Training	Initial Chemical Hygiene Awareness Training https://uky.scishield.com/node/2078577 Chemical Hygiene Annual Refresher Training https://uky.scishield.com/rafttraining/course/1346	Initial training at the time of hire or before involvement in this activity. Refresher training annually thereafter.
Works with chemicals requiring the use of a chemical fume hood as engineering control	Chemical Fume Hood Training**	Chemical Fume Hood Training https://ehs.uky.edu/classes/main.php?cClassID=22	Once, prior to beginning work in the chemical fume hood
Work includes perfusion or other procedures using formaldehyde	Formaldehyde Awareness Training	Formaldehyde Awareness Training https://ehs.uky.edu/classes/main.php?cClassID=128	Once, prior to beginning work with formaldehyde
Work in the lab produces chemical waste	Hazardous Waste Training	Hazardous Waste Training https://ehs.uky.edu/classes/main.php?cClassID=8	Initial training at the time of hire or before involvement in this activity. Refresher training annually thereafter.
Works with biohazards* in the laboratory	Biological Safety Training Dual Use Research of Concern (DURC) Training [Principal Investigators only]	Biological Safety Training https://uky.scishield.com/rafttraining/course/1344 Dual Use Research of Concern (DURC) Training https://uky.scishield.com/rafttraining/course/1345	Initial training at the time of hire or before involvement in this activity. Refresher training required every 3 years. Once
Works with biohazardous materials primarily associated with plants and/or plant pathogens (this course may be taken course in lieu of Biological Safety training.)	Plant Biosafety Training	Plant Biosafety Training https://uky.scishield.com/rafttraining/course/1322	Initial training at the time of hire or before involvement in this activity. Refresher training required every 3 years.
Works with human blood, bodily fluids, cells and tissues in the laboratory	Bloodborne Pathogens Training	Bloodborne Pathogens Training https://uky.scishield.com/rafttraining/course/1324	Initial training at the time of hire or before involvement in this activity. Refresher training annually thereafter.
Operates an autoclave as part of laboratory duties	Autoclave Training	Autoclave Training https://uky.scishield.com/rafttraining/course/1342	Once
Utilizes recombinant technology/viral vectors in the laboratory	Viral Vector Training	Viral Vector Training https://uky.scishield.com/rafttraining/course/1332	Once
Conducts procedures required to be performed in a biological safety cabinet (BSC)	Biological Safety Cabinet Training	Biological Safety Cabinet Training https://uky.scishield.com/rafttraining/course/589	Once
Works with radioactive materials	Radiation Safety Training	Initial Radiation Safety Training Register for this live training here Basic Radiation Training Register for this live training here Advanced Radiation Safety Training https://uky.scishield.com/rafttraining/course/1347	Once (prior to beginning work) Once (after completion of Initial Radiation Safety Training) Once (prior to being approved as an Authorized User)
Works with x-ray producing devices	X-ray Safety Training	Analytical X-ray Safety https://uky.scishield.com/rafttraining/course/102 Live training is also available - register here	Once
Works with Class IIIb or IV lasers	Laser Safety Training	Laser Safety Training https://ehs.uky.edu/classes/main.php?cClassID=16	Once (upon registration and prior to use of Class IIIb or IV lasers)
Works with materials or conducts procedures requiring the use of respiratory protection***	Respirator Training	Respirator Use Training https://ehs.uky.edu/classes/main.php?cClassID=25	Initially, prior to beginning work, then annually thereafter
Work includes shipment of dangerous goods off site. Dangerous good as defined by the U.S> Department of Transportation include explosives, compressed gases, flammable liquids and gases, corrosives, oxidizers, reactives, acute toxins, infectious substances and radioactive materials	DOT/IATA Training	DOT/IATA Training https://ehs.uky.edu/classes/classes_env_0001.php#dot_iata DOT/IATA Recertification Training https://ehs.uky.edu/classes/classes_env_0001.php#dot_iata_recertification	Once Every two years from date of initial training
Work procedures involve the use of Uranyl Acetate and/or Thorium	Uranyl Acetate and Thorium Awareness	Uranyl Acetate and Thorium Awareness https://uky.scishield.com/rafttraining/course/1348	Once

*Biohazardous materials may include but are not limited to

Recombinant nucleic acid molecules (ex. plasmids with inserts, viral vectors, etc.)
Synthetic nucleic acid molecules, including those that are chemically or otherwise modified but can base pair with naturally occurring nucleic acid molecules (ex. antisense oligonucleotides)
Whole animals and plants with introduced recombinant or synthetic nucleic acid molecules
Cells, organisms, and viruses containing recombinant or synthetic nucleic acid molecules
Infectious agents (bacterial, viral, fungal, parasitic, or prion) affecting humans, animals or plants
Infected animal blood and/or tissues
Human blood, blood products, or fluids
Human derived cell lines or tissues (including commercially acquired immortal cell lines)
Live vaccines

**Not required at this time, but strongly encouraged

*** Respirator users shall be enrolled in a Respiratory Protection Program for this work. Please contact UK Occupational Health and Safety (859.257.2924) for more information.

Physical Hazards

Biohazardous Materials

Institutional Biosafety Committee

Laboratory Equipment

Chemical Compatibility and Storage

Chemical Hygiene Plan

Chemical Inventory

Chemical Hazards Information

Laboratory Equipment

Policies and Procedures

Procedures and Processes

Radioactive Materials

X-Ray-Producing Devices

Other Resources

Training Aids

Training Requirements for Personnel Working in Research Wet Labs

The trainings listed below are required of all personnel working in wet labs conducting research at the University of Kentucky.

Job Activity

Safety Training Requirement

Training Method – Course Name

Training Frequency